Antidiabetic and antioxidant activity of Ocimum basilicum and Gundelia tournefortii extracts in a mouse modelرسالة ماجستير

Abstract

This thesis discusses chronic diabetes, especially type II diabetes, where the disease and its types were introduced in the introduction of the letter, as well as the introduction of Ocimum basilicum and Gundelia tournifortii plants, which were used to examine the effectiveness of their extracts at the blood sugar level through the use of experimental mice, and dividing mice into different groups (a healthy group, a healthy group received extract, a group with diabetes, a group with diabetes and received extract). With consideration of the ethics of dealing with animals, provide appropriate conditions for their breeding, and make the experiment. The disease was triggered by the use of streptozotocin with a dose of 50 mg/kg body weight, a diabetogenic agent for Type II diabetes, through injections into the abdomen and the destruction of Type B pancreatic cells. The mice received the extract via the gavage. During the experiment, the presence of any behavioral changes was monitored, in addition to monitoring weight, food, and water consumption, noting the difference in urination between groups, as it increases significantly in groups with diabetes compared to the other. The two plants were also evaluated as antioxidant plants by examining melanoaldehyde in several organs. Lastly, an assessment was conducted to see how well the plant affected the blood sugar levels of both diabetic and healthy mice. There was no significant effect (p≤0.05) of both plant extracts in biochemical parameter such as lowering blood sugar, Weight loss, consumption of food and water, our results were not approved with previous studies on the two plants at the cell level, as the extract of both plants succeeded to enhance the translocation of GLUT4 to the plasma membrane of L6myc muscle cells.