Please use this identifier to cite or link to this item: http://repository.aaup.edu/jspui/handle/123456789/1464
Title: Anti-Inflammatory, Antimicrobial, and Vasoconstriction Activities of an Anti-Hemorrhoidal Mixture of Alchemilla vulgaris, Conyza bonariensis, and Nigella sativa: In Vitro and Clinical Evaluations
Authors: Said, Omar$Other$Other
Khamaysi, Iyad $Other$Other
Kmail
Sadiq
Saied, Besan $Other$Other
Fulder, Stephen$Other$Other
Abofarekh, Basheer $Other$Other
Masalha, Mahmud $Other$Other
Amin, Riyad $Other$Palestinian
Saad
Keywords: morrhoidal disease
anti-inflammatory
vasoconstriction
Nigella sativa
Conyza bonar iensis
Alchemilla vulgar
Issue Date: 26-Jan-2022
Publisher: MDPI
Citation: Said, O.; Khamaysi, I.; Kmail, A.; Sadiq, O.; Saied, B.; Fulder, S.; Abofarekh, B.; Masalha, M.; Amin, R.; Saad, B. Anti-Inflammatory, Antimicrobial, and Vasoconstriction Activities of an Anti-Hemorrhoidal Mixture of Alchemilla vulgaris, Conyza bonariensis, and Nigella sativa: In Vitro and Clinical Evaluations. Immuno 2022, 2, 132–150. https://doi.org/ 10.3390/immuno2010010
Abstract: Abstract: Nigella sativa, Conyza bonariensis, and Alchemilla vulgaris are highly recommended in Greco Arab traditional medicine as anti-hemorrhoid medicinal plants. The efficacy and safety of a topical cream (HPC) consisting of water–ethanol extracts of these three plants were evaluated here in vitro and in a randomized, double-blind, placebo-controlled study (RDBPC). HPC showed no significant cytotoxic effects in fibroblast cell line 3T3 (LDH-release and MTT assay); it inhibited the nitric oxide production by cultured monocyte cell line THP-1 in a dose-dependent manner (reaching the control levels of untreated cells at a concentration of 100 µg/mL). HPC showed a dose-dependent antibacterial activity against Escherichia coli (60% inhibition compared to Ampicillin at 5 mg/disc) and a significant vasoconstriction effect on intestinal vein rings (40% increase compared to phenylephrine). In a RDBPC with 77 hemorrhoidal disease (patients ages 19–61 years with a median grade of hemorrhoids of 2.0), we determined the anti-hemorrhoid efficacy and safety of HPC. The patients were randomly assigned to the HPC group (54 patients) or the placebo group (23 patients). They were asked to apply 2–3 mL of HPC or placebo twice daily for 6 days. The degree of hemorrhoidal disease severity, hemorrhage severity, pain, and itching served as an evaluation of the HPC efficacy. Compared to the placebo group, the obtained results showed that 6 days of treatment with HPC reduced the indexes of hemorrhage severity, severity of pain, and severity of itching to 0–1, 1, and 1 after 6 days, respectively. In conclusion, patients treated with HPC had a significant clinical improvement in all disease severity parameters compared to placebo. In vitro evaluations proved HPC to have significant antimicrobial, anti-inflammatory, and vasoconstriction effects. Therefore, HPC represents an interesting alternative treatment for hemorrhoidal disease.
URI: http://repository.aaup.edu/jspui/handle/123456789/1464
Appears in Collections:Faculty & Staff Scientific Research publications

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