The Behavior of Ciprofloxacin at a DNA Modified Glassy Carbon Electrodes

Abstract

The voltammetric behavior of ciprofloxacin was investigated using cyclic voltammetry and differential-pulse anodic stripping voltammetry at bare glassy carbon (GC) electrodes and DNA-modified glassy carbon (DNA-GC) electrodes. For both types of electrodes, only one anodic irreversible wave was observed. A comparison between the current responses for the ciprofloxacin at the modified DNA-GC and unmodified GC electrodes, it was showed that the DNA-modified electrode exhibits a significant enhancement of the voltammetric current response with a better peak shape. Also, the interaction of ciprofloxacin with DNA was studied by using cyclic voltammetry technique at (DNA-GC) electrodes,which showed a weak interaction with a binding constant (K) = 2.89 x 105 M-1. A linear relationship between the peak current and ciprofloxacin concentrations was observed in the range 1.0–10.0 ?M, with a slope a detection limit of 0.117 ?M, with r = 0.998, and 1.0 ?M.