Please use this identifier to cite or link to this item: http://repository.aaup.edu/jspui/handle/123456789/1543
Title: Phytochemical analysis, cytostatic, cytotoxic, and anti-inflammatory effects of Arum palaestinum, Ocimum basilicum, and Trigonella foenum-graecum in human monocytic cell line (THP-1)-derived macrophages
Authors: Kmail, Abdalsalam O.$AAUP$Palestinian
Jaradat, Nidal$Other$Palestinian
Mansour, Bayan$AAUP$Palestinian
Abu-Labdeh, Ruhuf$AAUP$Palestinian
Zakarneh, Sondos$AAUP$Palestinian
Abu-Farha, Sawsan$AAUP$Palestinian
Hussein, Fatima$Other$Palestinian
Issa, Linda$Other$Palestinian
Saad, Bashar$AAUP$Palestinian
Keywords: Cancer
anti-inflammatory
Antioxidant
Medicinal plant
Herbal medicine
Trigonella foenum-graecum
Arum palaestinum
Ocimum basilicum
Issue Date: Sep-2022
Publisher: ELSEVIER
Citation: Abdalsalam Kmail, Nidal Jaradat, Bayan Mansour, Ruhuf Abu-Labdeh, Sondos Zakarneh, Sawsan Abu-Farha, Fatima Hussein, Linda Issa, Bashar Saad, Phytochemical analysis, cytostatic, cytotoxic, and anti-inflammatory effects of Arum palaestinum, Ocimum basilicum, and Trigonella foenum-graecum in human monocytic cell line (THP-1)-derived macrophages, European Journal of Integrative Medicine, Volume 54, 2022, 102159, https://doi.org/10.1016/j.eujim.2022.102159. (https://www.sciencedirect.com/science/article/pii/S1876382022000609)
Abstract: Introduction The significance of the crosstalk between pro-inflammatory mediators and carcinogenesis is widely discussed. These mediators play decisive roles at different stages of tumor development, including initiation, promotion, and metastasis. Arum palaestinum Boiss., Ocimum basilicum L., and Trigonella foenum-graecum L. and their crude extracts are traditionally used in the Arab and Islamic herbal medicine to treat a variety of cancers and inflammatory illnesses. Methods Human monocytic cell line (THP-1)-derived macrophages were used to evaluate anti-inflammatory, cytotoxic, and cytostatic effects of the ethanolic plant extracts. Cytotoxic and cytostatic effects were measured with the MTT assay. In addition, the production levels of pro-inflammatory mediators (TNF-α, IL-6, and nitric oxide) and anti-inflammatory cytokine (IL-10) were measured in lipopolysaccharide (LPS)-activated THP-1-derived macrophages in the absence and presence of increasing concentrations of the three plant extracts. Results The three plant extracts suppressed the production of NO and TNF-α and IL-6, and enhanced the production of IL-10 in LPS-activated THP-1-derived macrophages. In addition, these extracts inhibited the growth of THP-1-derived macrophages in a concentration-dependent manner at nontoxic concentrations. T. foenum-graecum exhibited the highest cytostatic effects with an IC50 of 512 µg/ mL compared to O. basilicum (no cytostatic effects) and A. palaestinum (IC50=1274 µg/mL). Conclusion Even though more studies are needed to elucidate the mechanisms of observed cytostatic and anti-inflammatory effects, to some extent, these effects could be attributed to the flavonoids, phenolic compounds, and tannin content detected in the plants’ extracts.
Description: https://elsevier.proofcentral.com/en-us/landing-page.html?token=a3aae0o812eca00f950fac9500338f
URI: http://dspacetest.aaup.edu/jspui/handle/123456789/1543
Appears in Collections:Faculty & Staff Scientific Research publications

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